REGULATION OF M2-TYPE PYRUVATE-KINASE FROM HUMAN MENINGIOMA BY ALLOSTERIC EFFECTORS FRUCTOSE 1,6 DIPHOSPHATE AND L-ALANINE

MELLATI A., YUCEL M., ALTINORS N., GUNDUZ U.

CANCER BIOCHEMISTRY BIOPHYSICS, cilt.13, sa.1, ss.33-41, 1992 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 1
  • Basım Tarihi: 1992
  • Dergi Adı: CANCER BIOCHEMISTRY BIOPHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.33-41
  • Anahtar Kelimeler: PYRUVATE KINASE, MENINGIOMA, ALLOSTERIC REGULATION, MULTIMOLECULAR FORMS, PURIFICATION, GLIOMAS, RAT, ISOENZYMES, INHIBITION, ISOZYMES, TYPE-M1, BRAIN
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

In the present study the mechanism of action of M2-type pyruvate kinase from human meningioma in the simultaneous presence of fructose 1,6 diphosphate and L-alanine was investigated. Purified pyruvate kinase from human meningioma was allosterically inhibited by L-alanine with respect to substrates phosphoenolpyruvate and ADP. The inhibitory effects of L-alanine was partially removed by fructose 1,6 diphosphate. The purified enzyme was slightly susceptible to ATP inhibition.