Influence of pH on recombinant human growth hormone production by Pichia pastoris

ÇALIK, PINAR; Bayraktar, Eda; Inankur, Bahar; Soyaslan, Elif; Sahin, Merve; Taspinar, Hatice; Acik, Eda; Yilmaz, Remziye; Ozdamar, Tuncer

doi:10.1002/jctb.2474

Influence of pH on recombinant human growth hormone production by Pichia pastoris

ÇALIK P. , Bayraktar E., Inankur B., Soyaslan E. S. , Sahin M., Taspinar H., ...More

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.85, no.12, pp.1628-1635, 2010 (Journal Indexed in SCI)

Publication Type: Article / Article
Volume: 85 Issue: 12
Publication Date: 2010
Doi Number: 10.1002/jctb.2474
Title of Journal : JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Page Numbers: pp.1628-1635
Keywords: Pichia pastoris, pH, sorbitol, gene expression, AOX, human growth hormone, BACILLUS-LICHENIFORMIS, STRUCTURAL-ANALYSIS, PROTEASE PRODUCTION, EXPRESSION SYSTEM, FERMENTATION, YEAST, BATCH, SECRETION, PURIFICATION, PROTEINS

Abstract

BACKGROUND: Effect of pH on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) was investigated at pH = 4.2, 5.0, 5.5, and 6.0.