Influence of pH on recombinant human growth hormone production by Pichia pastoris

ÇALIK P., Bayraktar E., Inankur B., Soyaslan E. S., Sahin M., Taspinar H., ...Daha Fazla

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.85, sa.12, ss.1628-1635, 2010 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85 Sayı: 12
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1002/jctb.2474
  • Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1628-1635
  • Anahtar Kelimeler: Pichia pastoris, pH, sorbitol, gene expression, AOX, human growth hormone, BACILLUS-LICHENIFORMIS, STRUCTURAL-ANALYSIS, PROTEASE PRODUCTION, EXPRESSION SYSTEM, FERMENTATION, YEAST, BATCH, SECRETION, PURIFICATION, PROTEINS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

BACKGROUND: Effect of pH on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) was investigated at pH = 4.2, 5.0, 5.5, and 6.0.