Influence of pH on recombinant human growth hormone production by Pichia pastoris

ÇALIK P., Bayraktar E., Inankur B., Soyaslan E. S., Sahin M., Taspinar H., ...More

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.85, no.12, pp.1628-1635, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 12
  • Publication Date: 2010
  • Doi Number: 10.1002/jctb.2474
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1628-1635
  • Keywords: Pichia pastoris, pH, sorbitol, gene expression, AOX, human growth hormone, BACILLUS-LICHENIFORMIS, STRUCTURAL-ANALYSIS, PROTEASE PRODUCTION, EXPRESSION SYSTEM, FERMENTATION, YEAST, BATCH, SECRETION, PURIFICATION, PROTEINS
  • Middle East Technical University Affiliated: Yes

Abstract

BACKGROUND: Effect of pH on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) was investigated at pH = 4.2, 5.0, 5.5, and 6.0.