Poly( amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering


Svobodova J., Proks V., Karabiyik O., Koyuncu A. C. C. , Kose G. T. , Rypacek F., ...More

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol.11, no.3, pp.831-842, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 3
  • Publication Date: 2017
  • Doi Number: 10.1002/term.1982
  • Title of Journal : JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
  • Page Numbers: pp.831-842
  • Keywords: poly( amino acid), fibrous scaffolds, adhesion peptide, radio-assay, HTGSC, MESENCHYMAL STEM-CELLS, HUMAN BONE-MARROW, PORE-SIZE, ADHESION PEPTIDES, RGD-PEPTIDES, GROWTH, CHITOSAN, POLYPEPTIDES, ATTACHMENT, HYDROGELS

Abstract

In this study, fibrous scaffolds based on poly(gamma-benzyl-L-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 mu m were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N-5-hydroxyethyl-L-glutamine units; and second, modification with the short peptide sequence azidopentanoyl-GGGRGDSGGGY-NH2, using the 'click' reaction on the previously modified scaffold with 2-propynylside-chains, was performed. Radio-assay of the I-125-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds ( which varied in the range 10(-3) -10 pM/cm(2)). All the PBLG scaffolds, especially with density 90 +/- 20 fM/cm(2) and 200 +/- 100 fM/cm(2) RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs. Copyright (C) 2015 John Wiley & Sons, Ltd.