Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus


Pekgoz G., GÜNDÜZ U. , Eroglu I. , Yucel M., Kovacs K., Rakhely G.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, cilt.36, ss.13536-13546, 2011 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 36 Konu: 21
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.ijhydene.2011.07.123
  • Dergi Adı: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Sayfa Sayısı: ss.13536-13546

Özet

Hydrogen production by nitrogenase is an energetically expensive process for the cell, hence strictly controlled at different levels. Ammonium is one of the substances regulating nitrogenase activity. The key proteins in the regulation of nitrogenase by ammonium are two regulatory proteins; GlnB and GlnK. In order to increase hydrogen production of Rhodobacter capsulatus DSM1710 (wild type strain) grown on agricultural materials/wastes, ammonium inhibition of nitrogenase enzyme has to be eliminated. In this study, GlnB and GlnK were targeted to be inactivated by in frame site-directed mutagenesis. The glnB mutant R. capsulatus (GP1 strain) was obtained at the end of mutagenesis studies. In the case of glnK, the suicide vector was constructed and delivered into the cells. However, glnK mutant could not be obtained.