Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus


Pekgoz G., GÜNDÜZ U., Eroglu I., Yucel M., Kovacs K., Rakhely G.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.36, no.21, pp.13536-13546, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 21
  • Publication Date: 2011
  • Doi Number: 10.1016/j.ijhydene.2011.07.123
  • Journal Name: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.13536-13546
  • Keywords: Rhodobacter capsulatus, Biohydrogen, GlnB/GlnK proteins, Ammonium inhibition, Site-directed mutagenesis, Kinetic modelling, P-II PROTEIN, HYDROGEN-PRODUCTION, NITROGEN-FIXATION, RHODOSPIRILLUM-RUBRUM, GLUTAMINE-SYNTHETASE, SPHAEROIDES, GLNK, ACCUMULATION, GENERATION, SELECTION
  • Middle East Technical University Affiliated: Yes

Abstract

Hydrogen production by nitrogenase is an energetically expensive process for the cell, hence strictly controlled at different levels. Ammonium is one of the substances regulating nitrogenase activity. The key proteins in the regulation of nitrogenase by ammonium are two regulatory proteins; GlnB and GlnK. In order to increase hydrogen production of Rhodobacter capsulatus DSM1710 (wild type strain) grown on agricultural materials/wastes, ammonium inhibition of nitrogenase enzyme has to be eliminated. In this study, GlnB and GlnK were targeted to be inactivated by in frame site-directed mutagenesis. The glnB mutant R. capsulatus (GP1 strain) was obtained at the end of mutagenesis studies. In the case of glnK, the suicide vector was constructed and delivered into the cells. However, glnK mutant could not be obtained.