Surface Water-Borne Multidrug and Heavy Metal-Resistant Staphylococcus Isolates Characterized by 16S rDNA Sequencing

Yilmaz F., Orman N., Serim G., Kochan C., Ergene A., İÇGEN B.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, cilt.91, sa.6, ss.697-703, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 91 Sayı: 6
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1007/s00128-013-1112-6
  • Dergi Adı: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.697-703
  • Anahtar Kelimeler: Staphylococcus, MRSA, CNS, 16S rDNA, COAGULASE-NEGATIVE STAPHYLOCOCCI, WASTE-WATER, ANTIBIOTIC-RESISTANCE, AQUATIC ENVIRONMENT, EVOLUTION, AUREUS, BACTERIA
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Four Staphylococcus isolates having both multidrug- and multimetal-resistant ability were isolated from surface water. Further identification of the isolates was obtained through biochemical tests and 16S rDNA gene sequencing. One methicillin-resistant and two methicilin-sensitive isolates were determined as Staphylococcus aureus. The other isolate was identified as Staphylococcus warneri. The antibiotic and heavy metal resistance profiles of the Staphylococcus isolates were determined by using 26 antibiotics and 17 heavy metals. S. aureus isolates displayed resistance to most of the beta-lactam antibiotics tested. All Staphylococcus isolates were resistant to heavy metals including silver, lithium, and barium. Due to a possible health risk of these pathogenic bacteria, a need exists for an accurate assessment of their acquired resistance to multiple drugs and metals.