Estimation of phytoplankton biomass using HPLC pigment analysis in the southwestern Black Sea

Ediger D., Soydemir N., Kideys A. E.

DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, cilt.53, ss.1911-1922, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.dsr2.2006.04.018
  • Dergi Adı: DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1911-1922
  • Anahtar Kelimeler: phytoplankton composition, microscopy, photosynthetic pigments, chemotaxonomy, Black Sea, PERFORMANCE LIQUID-CHROMATOGRAPHY, UNICELLULAR MARINE-ALGAE, CHLOROPHYLL-A, SPRING BLOOM, OCEAN, FINGERPRINTS, VARIABILITY, SIGNATURES, PROFILES, ATLANTIC
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The phytoplankton population of the southwestern Black Sea in May 2001 was studied by taxonomic analysis using microscopic examination and by pigment analyses using high-performance liquid chromatography (HPLC). Pigment data, which identified phytoplankton assemblages dominated by dinoflagellates, diatoms and coccolithophores in May 2001, were compared to phytoplankton cell counts and biomass. There were significant (p < 0.002-0.01, r=0.56-0.67) relationships between the taxon-specific pigment concentrations and the taxon-specific cell numbers during this sampling period. The ratios of chlorophyll-a to the dominant accessory pigments calculated by multiple linear regressions were 1.2 (chlorophyll-a: peridinin) in dinoflagellates, 1.8 (chlorophyll-a: fucoxanthin) in diatoms, and 2.66 (chlorophyll-a: 19'-hex on oyloxyfucoxanthin) in coccolithophores. HPLC-determined chlorophyll-a biomass correlated well with the sum of the group-specific pigment biomass (p < 0.001, r(2) = 0.95). The phytoplankton assemblage as revealed by the microscopic and HPLC analyses was thus made up of common Black Sea groups showing that HPLC pigment analysis can be used to quantify phytoplankton assemblages in the Black Sea based on simple ratios. (c) 2006 Elsevier Ltd. All rights reserved.