RAPID AND COMPREHENSIVE SEPARATION FOR THE PHENOLIC CONSTITUENTS OF QUERCUS BRANTII ACORNS BY RP-HPLC-DAD

ÇORUH N., Nebigil C., Ozgokce F.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol.37, no.6, pp.907-915, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 6
  • Publication Date: 2014
  • Doi Number: 10.1080/10826076.2012.759126
  • Journal Name: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.907-915
  • Keywords: acorns, fractionation, HPLC-DAD, isolation, phenolic composition, Quercus brantii, ANTIOXIDANT, POLYPHENOLS, LEAVES, CORK, L.
  • Middle East Technical University Affiliated: Yes

Abstract

Quercus brantii Lindley (Fagaceae) is a common oak region of South and East Anatolia. For many civilizations, acorns of Quercus species have been considered as food as well as used in folk-medicine. Phenolic composition of the acorns of Q. brantii was studied by high performance liquid chromatography equipped with a diode array detector. To the best of our knowledge, this is the first study regarding a comprehensive separation and identification of the phenolic constituents of Q. brantii acorns. Rapid separation and identification were performed by the reverse phase HPLC-DAD. Identification of the phenolics was also confirmed by UPLC-MS and when necessary structural characterization was accomplished by NMR and FT-IR techniques. As a result, the phenolic composition of the acorns of Q. brantii were realized as gallic acid, methyl gallate, epi-catechin, p-coumaric acid, ellagic acid, rutin and quercetin in the respective elution order of the RP-HPLC chromatogram.