RAPID AND COMPREHENSIVE SEPARATION FOR THE PHENOLIC CONSTITUENTS OF QUERCUS BRANTII ACORNS BY RP-HPLC-DAD


ÇORUH N. , Nebigil C., Ozgokce F.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.37, ss.907-915, 2014 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 37 Konu: 6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/10826076.2012.759126
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Sayfa Sayıları: ss.907-915

Özet

Quercus brantii Lindley (Fagaceae) is a common oak region of South and East Anatolia. For many civilizations, acorns of Quercus species have been considered as food as well as used in folk-medicine. Phenolic composition of the acorns of Q. brantii was studied by high performance liquid chromatography equipped with a diode array detector. To the best of our knowledge, this is the first study regarding a comprehensive separation and identification of the phenolic constituents of Q. brantii acorns. Rapid separation and identification were performed by the reverse phase HPLC-DAD. Identification of the phenolics was also confirmed by UPLC-MS and when necessary structural characterization was accomplished by NMR and FT-IR techniques. As a result, the phenolic composition of the acorns of Q. brantii were realized as gallic acid, methyl gallate, epi-catechin, p-coumaric acid, ellagic acid, rutin and quercetin in the respective elution order of the RP-HPLC chromatogram.