Principles of Fluorescence Spectroscopy and Microscopy
- Dersin Düzeyi: Bütünleşik Doktora
- Tasarlanan Ders Kodu: 2380545
- Öğretim Türü: Örgün Öğretim (Normal Öğretim)
- Dersin Kapsamı: Teorik ve Uygulama
- Akademik Yıl: 2010 - 2011
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Ders İçeriği:
Course objectives: Throughout the course developments in the instrumentation and fluorescence labels will be introduced. Applications of the new fluorescence dyes and proteins to biological samples will be discussed. Case studies of new methods such as single molecule detection in living cells, fluorescence energy transfer techniques to identify protein-protein interactions and florescent biosensors will be introduced.
Course Outline:
Week 1. Introduction
Week 2. Instrumentation for Fluorescence Spectroscopy and applications to structural biology
Week 3. Biological and chemical fluorophores and quantum dots (QDots)
Week 4. Quenching of Fluorescence
Week 5. Confocal Microscopy and its applications to structural biology
Week 6. Super Resolution Microscopy and LightSheet Microscopy
Weeks 7-8. Fluorescence-resonance energy transfer (FRET) applications
Week 9. Fluorescence-Lifetime Imaging Microscopy
Week 10. Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Loss in Photobleaching (FLIP) and their applications to study protein dynamics
Week 11. Time-Resolved Fluorescence and Two-Photon Fluorescence Correlation Spectroscopy
Week 12. Total internal reflection fluorescence (TIRF) Microscopy
Week 13. Single-Molecule Detection
Week 14. Live cell imaging and use of fluorescence labels in real-time detection of protein dynamics