Akademik Veri Yönetim Sistemi
Course objectives: Throughout the course developments in the instrumentation
and fluorescence labels will be introduced. Applications of the new
fluorescence dyes and proteins to biological samples will be discussed. Case
studies of new methods such as single molecule detection in living cells,
fluorescence energy transfer techniques to identify protein-protein
interactions and florescent biosensors will be introduced.
Course Outline:
Week 1. Introduction
Week 2. Instrumentation for Fluorescence Spectroscopy and
applications to structural biology
Week 3. Biological and chemical fluorophores and quantum
dots (QDots)
Week 4. Quenching of Fluorescence
Week 5. Confocal Microscopy and its applications to
structural biology
Week 6. Super Resolution Microscopy and LightSheet
Microscopy
Weeks 7-8. Fluorescence-resonance energy transfer (FRET)
applications
Week 9. Fluorescence-Lifetime Imaging Microscopy
Week 10. Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Loss in Photobleaching (FLIP) and their applications to study protein
dynamics
Week 11. Time-Resolved Fluorescence and Two-Photon Fluorescence Correlation Spectroscopy
Week 12. Total internal reflection fluorescence (TIRF) Microscopy
Week 13. Single-Molecule Detection
Week 14. Live cell imaging and use of fluorescence
labels in real-time detection of protein dynamics