Design and synthesis of novel substituted indole-acrylamide derivatives and evaluation of their anti-cancer activity as potential tubulin-targeting agents

Hawash M., KAHRAMAN D. C., OLĞAÇ A., Ergun S. G., Hamel E., Cetin-Atalay R., ...Daha Fazla

JOURNAL OF MOLECULAR STRUCTURE, cilt.1254, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1254
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.molstruc.2022.132345
  • Dergi Adı: JOURNAL OF MOLECULAR STRUCTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chimica, Compendex, INSPEC
  • Anahtar Kelimeler: Synthesis, Anticancer activity, Indole, Tubulin polymerization, Colchicine binding, Cell cycle arrest, Molecular docking, BIOLOGICAL EVALUATION, ACCURATE DOCKING, POLYMERIZATION, INHIBITORS, BINDING, MICROTUBULES, EXPRESSION, PHOSPHATE, ANALOGS, GROWTH
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Novel compounds containing polar and nonpolar substitutions on the prop-2-en-1-on linker of the trans-indol-3-ylacrylamide scaffold were designed by modeling within the colchicine site of tubulin and then synthesized to determine the role of such substitutions on tubulin polymerization. We first determined the in vitro antiproliferation activities of the compounds against cancer cell lines with a particular focus on hepatocellular carcinoma. The results indicated that five of the compounds showed moderate antitumor activities. When the tubulin polymerization inhibitory effect of these compounds was evaluated, compound 13 was determined to be a tubulin polymerization inhibitor. Furthermore, cell cycle analysis for compound 13 resulted in G2/M-phase arrest in Huh7 cells. The results indicated that polar substitutions on the indole acrylamide scaffold enhance potency against tubulin polymerization. However, the substitution-related bioactivity shifting was not observed on the cancer cell lines since the inhibition mechanisms of the compounds may vary. (C) 2022 Elsevier B.V. All rights reserved.