Improvements in microstructural, mechanical, and biocompatibility properties of nano-sized hydroxyapatites doped with yttrium and fluoride


BAŞAR B., TEZCANER A., KESKİN D., EVİS Z.

CERAMICS INTERNATIONAL, vol.36, no.5, pp.1633-1643, 2010 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 5
  • Publication Date: 2010
  • Doi Number: 10.1016/j.ceramint.2010.02.033
  • Journal Name: CERAMICS INTERNATIONAL
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1633-1643
  • Keywords: Sintering, X-ray methods, Hardness, Apatite, Biomedical applications, ALKALINE-PHOSPHATASE ACTIVITY, NANOCRYSTALLINE HYDROXYAPATITE, ZIRCONIA COMPOSITES, IN-VITRO, SINTERED HYDROXYFLUORAPATITES, BIOMEDICAL APPLICATIONS, NANOPHASE CERAMICS, HYDROXYLAPATITE, BIOCERAMICS, TITANIUM

Abstract

Hydroxyapatite was doped with Y3+ (2.5, 5 and 7.5 mol%) and F (2.5 mol%) ions (2.5YFHA, 5YFHA, 7.5YFHA, respectively) to compare its structural and mechanical properties and cellular response with pure-hydroxyapatite. No second phases were observed by X-ray diffraction spectra of 2.5YFHA. Doped hydroxyapatites had F- bonds in addition to OH- bonds. Hydroxyapatites sintered at 900 and 1100 degrees C were in nano-size. 7.5YFHA sintered at 1300 degrees C had the highest microhardness value. 2.5YFHA sintered at 1100 degrees C had the highest fracture toughness value. MTT viability assays showed high cell attachments on 2.5YFHA. Cell proliferation on 2.5YFHA and 5YFHA sintered at 1100 and 1300 degrees C was comparable with the control after 5-day culture. The highest ALP production and calcium deposition were observed on all hydroxyapatites sintered at 1100 degrees C. 2.5YFHA sintered at 1100 degrees C can be an alternative for hydroxyapatite in orthopedic applications. (C) 2010 Elsevier Ltd and Techna Group S.r.l. All rights reserved.