Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

ÇALIK P., Bozkurt B., Zerze G. H., Inankur B., Bayraktar E., BOY E., ...Daha Fazla

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.88, sa.9, ss.1631-1640, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 88 Sayı: 9
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/jctb.4011
  • Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1631-1640
  • Anahtar Kelimeler: P.pastoris, human growth hormone, sorbitol, methanol, feeding rate, feeding strategy, ALCOHOL OXIDASE, CARBON-SOURCES, PROTEINS, METHANOL, SECRETION, BATCH
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.