Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis

Gurakan G. C., Bayindirli A., Ogel Z., Tanboga P.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.14, no.2, pp.293-295, 1998 (SCI-Expanded) identifier identifier


Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined.