IMMOBILIZATION OF BETA-GALACTOSIDASE ONTO GELATIN BY GLUTARALDEHYDE AND CHROMIUM(III) ACETATE

SUNGUR S., AKBULUT U.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.59, no.3, pp.303-306, 1994 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 59 Issue: 3
  • Publication Date: 1994
  • Doi Number: 10.1002/jctb.280590314
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Computer & Applied Sciences, EMBASE, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.303-306
  • Middle East Technical University Affiliated: Yes

Abstract

Beta-Galactosidase (EC 3.2.1.23) was immobilised onto a gelatin carrier system by crosslinking. Glutaraldehyde and chromium(III) acetate were used as crosslinking agents. The effect of pH on enzyme activity, kinetic parameters and reusability of the immobilised enzyme were investigated. To obtain a better relative activity, a method was developed and further modified to increase the surface area of the support system by using polyacrylamide. Relative activity enhancement of 68% was achieved in the gelatin-glutaraldehyde system.