Immobilization of yeast cells in several conducting polymer matrices
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, cilt.39, sa.3, ss.183-197, 2002 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 3
- Basım Tarihi: 2002
- Doi Numarası: 10.1081/ma-120013259
- Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.183-197
- Anahtar Kelimeler: immobilization, yeast, invertase, polypyrrole, poly(methyl methacrylate), SACCHAROMYCES-CEREVISIAE CELLS, INVERTASE ACTIVITY, HYDROLYSIS, MEMBRANE
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Immobilization of yeast cells (Saccharomyces cerevisiae) in different polymer matrices was performed by constant potential electrolysis. These matrices were polypyrrole (PPy); poly(methyl methacrylate)/polypyrrole (,PMMA/PPy) and thiophene-capped poly(methyl methacrylate)/ polypyrrole (TPMMA/PPy). The characterization of PMMA/PPy copolymer was achieved by Fourier-transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The invertase activity of immobilized yeast cells was determined. Optimum temperature, Michaelis-Menten constants and maximum reaction rates of the enzyme electrodes were compared with those of free yeast cells. The operational and storage stabilities of three different immobilization systems were analyzed.