Multifunctional layer-by-layer modified chitosan/poly(ethylene glycol) hydrogels

Onat B., Ulusan S., Banerjee S., Erel Göktepe İ.

EUROPEAN POLYMER JOURNAL, cilt.112, ss.73-86, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 112
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.eurpolymj.2018.12.028
  • Dergi Adı: EUROPEAN POLYMER JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.73-86
  • Anahtar Kelimeler: Chitosan hydrogel, Layer-by-layer, Temperature-responsive, Bioactive, Antibacterial, CHITOSAN-BASED HYDROGELS, BONDED MULTILAYERS, TANNIC-ACID, SURFACE MODIFICATION, FIBROBLAST ADHESION, PHYSICAL-PROPERTIES, RELEASE, POLYMER, GROWTH, CIPROFLOXACIN
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

We report the surface modification of chitosan/poly(ethylene glycol) (chitosan/PEG) hydrogel materials via layer-by-layer (LbL) technique using stimuli-responsive polymers. Water-soluble complexes of Tannic Acid (TA) and a broad-spectrum antibiotic, Ciprofloxacin (Cipro) were prepared and co-assembled at the surface of Chitosan/PEG hydrogels with poly(N-vinyl caprolactam) (PVCL). Compared to the bare hydrogels, the surface spreading and proliferation of human fibroblasts were significantly enhanced on precast hydrogels coated with TA-Cipro/PVCL multilayers. LbL coating also provided enhanced Cipro release from the hydrogel surface at 37 degrees C compared to bare hydrogels. TA-Cipro/PVCL coated hydrogels showed antibacterial activity through chitosan and temperature-induced release of Cipro from multilayers. Chitosan and Cipro showed a coordinated antibacterial effect on Eschericia coli and Bacillus cereus, reducing their minimum inhibitory concentration (MIC). This effect was more pronounced on B. cereus. LbL modification of chitosan-based hydrogels using stimuli responsive polymers can be advantageous for bringing multiple functionalities to these materials without sacrificing their intrinsic properties such as antibacterial activity and biocompatibility. Such LbL-coated hydrogels hold promise in wound treatment as they may promote fibroblast proliferation and skin tissue regeneration.