The effects of different methods of application of chlortetracycline and oxytetracycline on microbial properties of lamb meat were studied. A significant antibacterial effect of antibiotic was found on total mesophilic bacteria count. The mesophilic bacteria level was determined by two methods: the conventional method using Plate Count Agar and bioluminescence. Correlation curves of the CFU and RLU Values proved two methods were highly related. Thus for the microbiology quality of meat hygiene the bioluminescence method may be used as a fast and alternative method to PCA. Note that bioluminescence requires a higher degree of skill and attention.