GLUCOSE-OXIDASE IMMOBILIZED ON GELATIN BY VARIOUS CROSS-LINKERS
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, ss.137-147, 1993 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 1993
- Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.137-147
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Immobilization of glucose oxidase have been realized with photographic gelatin as support for chemical coupling. Chromium acetate, potassium chromium sulfate and formaldehyde were used as crosslinking agents to immobilize glucose oxidase on film strips. Enzyme, crosslinker concentrations, temperature, pH and reuse number on native and immobilized glucose oxidase activity and water contents of unused and used films were investigated. The best results were achieved with the crosslinker chromium acetate as 50% maximum activity after 40 assays.