STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING

SUNGUR S., ELCIN Y., AKBULUT U.

BIOMATERIALS, cilt.13, sa.11, ss.795-800, 1992 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 11
  • Basım Tarihi: 1992
  • Doi Numarası: 10.1016/0142-9612(92)90020-o
  • Dergi Adı: BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.795-800
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).