STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING

SUNGUR S., ELCIN Y., AKBULUT U.

BIOMATERIALS, vol.13, no.11, pp.795-800, 1992 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 11
  • Publication Date: 1992
  • Doi Number: 10.1016/0142-9612(92)90020-o
  • Journal Name: BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.795-800
  • Middle East Technical University Affiliated: Yes

Abstract

Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).