Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G

Erdemli O., Usanmaz A., Keskin D., Tezcaner A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.117, ss.487-496, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 117
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.colsurfb.2014.01.037
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.487-496
  • Anahtar Kelimeler: Protein delivery, Microspheres, Polymeric drug delivery, Methoxy poly(ethyleneglycol)-poly(epsilon-caprolactone)-methoxy poly(ethylene glycol), Amphiphilic triblock copolymer, gamma-sterilization, PLGA MULTIBLOCK COPOLYMERS, GLYCOL) BLOCK-COPOLYMERS, DRUG-DELIVERY SYSTEM, GAMMA-IRRADIATION, EPSILON-CAPROLACTONE, IN-VITRO, BIODEGRADABLE MICROSPHERES, POLY(ETHYLENE GLYCOL), THERMOSENSITIVE HYDROGEL, POLYMERIC MICROSPHERES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Polyester-polyether type block copolymers have attracted attention in the area of drug delivery systems with their capability in providing a broad range of amphiphilic characteristics. The aim of the present work was to prepare and characterize immunoglobulin G (IgG) loaded methoxy poly(ethylene glycol)-poly(epsilon-caprolactone)-methoxy poly(ethylene glycol) (MPEG-PCL-MPEG) microspheres as potential carrier for therapeutic monoclonal antibodies used in clinics. MPEG-PCL-MPEG triblock copolymer was synthesized by ring-opening polymerization of epsilon-caprolactone initiated by MPEG and then characterized. Microspheres were prepared by double emulsion-solvent evaporation method and their properties were compared with those of PCL microspheres. Microspheres had spherical shape with a mean particle size around 6 pm. MPEG-PCL-MPEG microspheres had higher encapsulation efficiency than PCL microspheres. After 90 days of release, 30 +/- 2% and 57 +/- 3% of the bioactivity of IgG released from non-irradiated PCL and MPEG-PCL-MPEG microspheres were protected, respectively. Presence of MPEG in microspheres provided more controlled IgG release rate and protected IgG from denaturation during gamma-irradiation (20 +/- 3% and 49 +/- 2% for PCL and MPEG-PCL-MPEG microspheres, respectively). In vitro cytotoxicity tests revealed that both MPEG-PCL-MPEG and PCL microspheres had no toxic effect on cells. This study showed that MPEG-PCL-MPEG microspheres are promising delivery systems for therapeutic monoclonal antibodies. (C) 2014 Elsevier B.V. All rights reserved.