Formation and Characterization of Green Tea Extract Loaded Liposomes


DAG D., Oztop M. H.

JOURNAL OF FOOD SCIENCE, cilt.82, sa.2, ss.463-470, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 82 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1111/1750-3841.13615
  • Dergi Adı: JOURNAL OF FOOD SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.463-470
  • Anahtar Kelimeler: encapsulation, green tea, liposome, microfluidization, ultrasonication, ANTIOXIDANT ACTIVITY, CATECHIN, MICROFLUIDIZATION, ENCAPSULATION, CHITOSAN, SCIENCE
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Green tea extract was encapsulated into liposomes to enhance bioavailability and stability of catechins by protecting their functional properties simultaneously. Encapsulation was achieved by dispersing 1% (w/v) soy lecithin through high pressure homogenization (microfluidization) and ultrasonication. Effects of homogenization type and pH of the dispersing medium on the physical properties and stability of the liposomes during 1-mo storage period were investigated. Mean particle size, total phenolic content by Folin-Ciocalteu method and antioxidant activity by 2-diphenyl-1-picrylhydrazyl radical scavenging and ferric reducing-antioxidant power methods, and transmission electron microscopy (TEM) experiments were conducted for characterization. Green tea extract loaded liposomes prepared by microfluidization in distilled water were determined as the most stable system which demostrated no significant difference (P > 0.05) on mean particle size, total phenolic content, and antioxidant activity between the first and final day of 1-mo storage time. Additionally, uniform size and shape in TEM images supported the results.