Production, purification and characterization of chitosanase from Penicillium spinulosum
BIOCHEMICAL ARCHIVES, cilt.14, sa.4, ss.221-225, 1998 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 14 Sayı: 4
- Basım Tarihi: 1998
- Dergi Adı: BIOCHEMICAL ARCHIVES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.221-225
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
A chitosan degrading fungus which had been isolated in our laboratory and identified as Penicillium spinulosum by the International Mycological Institute (England), was used for the production of chitosanase in a salt medium containing Rhizopus cell walls as the sole carbon source. Although chitosanase was produced under all the conditions tested about 1 % cell wall concentration maximized enzyme production. The enzyme was purified 50 fold by using ammonium sulfate precipitation and ion-exchange chromatography and then characterized. Optimal pH and temperature values of the enzyme were determined as pH 5 and 55 degrees C, respectively. The K-m and V-max values were found as 1.7 mg chitosan/ml and 146.4 mu mol glucosamine/ml-min, respectively by using Lineweaver-Burk plot. Approximately 15% and 50% activity losses were observed during a 20 minutes heat treatment at 45 and 65 degrees C at pH 4.2, respectively.