Design a cadA-targeted DNA probe for screening of potential bacterial cadmium biosorbents

İçgen B., Yılmaz F.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol.23, pp.5743-5752, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 23
  • Publication Date: 2016
  • Doi Number: 10.1007/s11356-015-5810-y
  • Journal Name: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.5743-5752
  • Keywords: Cadmium removal, Cadmium biosorbents, cadA, cadA probe, GENES, ZINC, IDENTIFICATION, EXPOSURE, REMOVAL
  • Middle East Technical University Affiliated: Yes

Abstract

Due to their metal removal ability, bacterial biosorbents can be effectively used for the treatment of wastewaters containing heavy metals. Searching for bacterial biosorbents for hazardous heavy metals like cadmium is a pivotal for remediation efforts. The gene cadA, that mediates resistance to cadmium over an ATP-dependent efflux mechanism, provides a good target for the selection of potential cadmium biosorbents. For this reason, in this study, a 36-mer-oligonucleotide DNA probe based on the entire 3.5-kb BglII-XbaI fragment of cadA operon from staphylococcal plasmid pI258 was prepared by using Vector NTI Express software. Under the hybridization conditions of 46 A degrees C, 50 % formamide, and 0.028 M NaCl, the designed cadA probe appeared to be highly specific to the cadA-positive Staphylococcus warneri and Delftia acidovorans isolates tested. The results indicated that the newly designed cadA-targeted DNA probe has potential as a specific, sensitive, and quantitative tool in selecting and in situ screening of potential cadmium biosorbents.