Carboligation reactions with benzaldehyde lyase immobilized on superparamagnetic solid support

Sopaci S. B., Turan I. S., Tural B., Volkan M., DEMİR A. G.

ORGANIC & BIOMOLECULAR CHEMISTRY, cilt.7, sa.8, ss.1658-1664, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 8
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1039/b819722a
  • Dergi Adı: ORGANIC & BIOMOLECULAR CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1658-1664
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Histidine-tagged recombinant benzaldehyde lyase (BAL, EC 4.1.2.38) was efficiently immobilized to surface-modified magnetic particles with affinity ligand binding. In addition to conventional benzoin condensation reactions, two important representative BAL-catalyzed carboligation reactions, were also performed with this magnetically responsive biocatalyst. The results obtained from the carboligation reactions that were performed with this simple and convenient heterogenous biocatalyst were comparable to that of free-enzyme-catalyzed reactions.