Multilayer fibroin/chitosan oligosaccharide lactate and pullulan immunomodulatory patch for treatment of hernia and prevention of intraperitoneal adhesion


Akbaba S., Atila D., KESKİN D., Tezcaner T., TEZCANER A.

Carbohydrate Polymers, vol.265, 2021 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 265
  • Publication Date: 2021
  • Doi Number: 10.1016/j.carbpol.2021.118066
  • Journal Name: Carbohydrate Polymers
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, PASCAL, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Hernia, Immunomodulation, Peritoneal adhesion, Regeneration, SILK FIBROIN HYDROGEL, SURFACE MODIFICATION, ABDOMINAL-WALL, RAW 264.7, IN-VITRO, POLYPROPYLENE MESH, PERITONEAL-FLUID, POLYSACCHARIDE, COMPOSITE, DERIVATIVES

Abstract

© 2021 Elsevier LtdThis study aims to develop a novel intraperitoneal two- or three-layered patch with immunomodulatory property for treatment of hernia, regeneration of abdominal wall and prevention of intraperitoneal adhesions. Polypropylene (PP) mesh, middle layer, was intended to provide mechanical support whereas pullulan (PUL) hydrogel coating layer was designed to prevent intraperitoneal adhesions. Fibroin/chitosan oligosaccharide lactate (F/COS) layer electrospun on one side of pullulan was chosen for immunomodulation and abdominal wall regeneration. Physical and mechanical properties and regenerative capacity of intraperitoneal patches were determined. Immunomodulatory property of electrospun layer and whole patch was studied by determining nitric oxide amount produced by RAW 264.7 macrophages. 25 % (w/v) PUL hydrogel and F/COS with 90:10 (w/w) ratio yielded optimal results. Here, we report that fabricated intraperitoneal patches successfully prevented cell adhesion on one side and increased cell viability and proliferation on other side, along with immunomodulation, in vitro.