The present study pertains to the influence of variation of FRP (Fiber Reinforced Polymer) proportion in tension reinforcement on the flexural behavior of RC beams with FRP and steel reinforcing bars. A total of 25 beams, including FRP-, steel- and hybrid FRP-steel reinforced ones, were tested to failure under four-point bending. Two types of FRP bars, GFRP (Glass Fiber Reinforced Polymer) and BFRP (Basalt Fiber Reinforced Polymer), were used and both over- and under-reinforced beams were tested. The beams in each group were designed to have close flexural capacities to fully reveal the effect of FRP proportion in the tension zone on beam ductility for a fixed bending capacity. A new analytical model was developed for estimating the bending capacities of beams. Different deformation and curvature ductility definitions were adopted and an energy-based definition, revealing the expected tendency in beam ductility, was determined. The test results revealed that the presence of even a single FRP bar in the tension zone results in reductions up to 40% in beam ductility as compared to the beam with full steel reinforcement. Each additional replacement of a steel bar with FRP was found to cause a further decrease up to 20% in beam ductility.