Assessment and optimization of xylanase production using co-cultures of Bacillus subtilis and Kluyveromyces marxianus

Yardimci G. O., ÇEKMECELİOĞLU D.

3 BIOTECH, cilt.8, sa.7, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 7
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s13205-018-1315-y
  • Dergi Adı: 3 BIOTECH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Xylanase, Optimization, Co-culture, Submerged fermentation, Bacillus subtilis, Kluyveromyces marxianus, CELLULASE-FREE XYLANASE, ASPERGILLUS-NIGER, SUBMERGED FERMENTATION, MIXED CULTURE, PURIFICATION, PARAMETERS, ENZYMES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Co-cultures of Bacillus subtilis and Kluyveromyces marxianus were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved with 4% B. subtilis, 4% K. marxianus, 40% solid load of hazelnut shells, and pH 7.0. Overall, xylanase was enhanced by 4.4-fold compared to initial un-optimized monoculture production and 2.8-fold compared to initial un-optimized co-cultured production, after optimization by response surface method.