intI1 Type Mobile Genetic Elements Co-selected Antibiotic-Resistant Genes in Untreated Hospital Wastewaters

Kayali O., İçgen B.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol.106, no.2, pp.399-405, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 106 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.1007/s00128-020-03098-z
  • Journal Name: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Greenfile, INSPEC, MEDLINE, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.399-405
  • Keywords: ARG, HWW, MGE, intI1, ISCR1, Tn916/tn1545, EMERGENCE, BACTERIA, CLASS-1
  • Middle East Technical University Affiliated: Yes

Abstract

Dissemination of antibiotic-resistant genes (ARGs) from hospital wastewaters (HWWs) is facilitated by the horizontal gene transfer (HGT) and involves association of ARGs with mobile genetic elements (MGEs). In our previous study, HWWs were found to have relatively high copy numbers of ARGs aadA, tetA, cmlA, sul1, and qnrS. In this study, therefore, the same HWWs were also monitored for 3 MGEs class 1 integron (intI1), insertion sequence common region 1 (ISCR1) and conjugative transposon Tn916/Tn1545 by using quantitative polymerase chain reaction. The gene intI1 with 7.4 x 10(2) average copy number/mL was found to be the most prevalent MGE and was up to two orders of magnitude higher than ISCR1 (5.5 x 10(0) average copy number/mL, p < 0.05) and Tn916/Tn1545 (2.3 x 10(0) average copy number/mL, p < 0.05) in all HWWs tested. Positive correlation between intI1 and the aadA, tetA, cmlA and sul1 genes indicated that the MGEs harbouring class1 integron most likely played major role in co-selecting all these ARGs together.