Sequence specific inhibition of human chemokine gene, CXCL7, by small interfering rnas in lewis lung carcinoma cells

DİLAY KIZIŞAR

Sequence specific inhibition of human chemokine gene, CXCL7, by small interfering rnas in lewis lung carcinoma cells

Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2013

Öğrenci: DİLAY KIZIŞAR

Danışman: MAHİNUR AKKAYA

Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu

Özet:

In this study, we aimed to design small interfering RNAs to silence the expression of a gene that is a member of chemokines playing crucial roles on cancer. Small interfering RNAs (siRNAs) are generated by ribonuclease III cleavage of 20-25nt in length dsRNAs which mediate RNAi-induced specific mRNA degradation and they are known to be an important part of the gene regulation mechanism in all Eukaryotes. Recently, siRNAs are being utilized as promising treatment approaches. In this study, we targeted a gene involved in lung cancer CXCL7 via designing potent siRNA sequences and carried out optimization for efficient transfection. The level of silencing in mouse cell culture was determined using a Flow Cytometry.