Investigation of SNX3 in colon cancer

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyolojik Bilimler Bölümü, Türkiye

Tezin Onay Tarihi: 2019

Öğrenci: HARUN CİNGÖZ

Danışman: AYŞE ELİF ERSON BENSAN

Özet:

Sorting Nexin 3 (SNX3) is part of the retromer complex that recycles cargo receptors back to plasma membrane or to Trans Golgi Network. WNT ligand carrier protein Wntless (WLS) is a known SNX3 cargo protein. Our earlier data suggested over expression of SNX3 in colon cancer cells. Considering its importance in receptor recycling, we hypothesized SNX3 to be a potential modulator of cancer related receptors. To begin understanding the role of SNX3, we developed RNAi models of SNX3 in SW480 colon cancer cells and investigated biochemical and phenotypical outcomes of SNX3 knock down. Here we present evidence that SNX3 might regulate several receptors important in colon cancers and decreased SNX3 expression alters motility and migration of colon cancer cells by downregulating important signaling pathways with implications in epithelial to mesenchymal transition.