Multi-view subcellular localization prediction of human proteins

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2019

Tezin Dili: İngilizce

Öğrenci: GÖKHAN ÖZSARI

Danışman: Mehmet Volkan Atalay

Özet:

Determining the subcellular localization of proteins is crucial for Understanding the functions of proteins, drug targeting, systems biology, and proteomics research. Experimental validation of subcellular localization is an expensive and challenging process. There exist several computational methods for automated prediction of protein subcellular localization; however, there is still room for better performance. Here, we propose a multi-view SVM-based approach that provides predictions for human proteins. We represent each protein sequence by multi-view features; i.e., physicochemical properties, amino acid compositions, and homology-based features. Our classification model contains seven classifiers for each localization, where each classifier provides a probabilistic result. To develop a multi-view voting classifier, we employ a weighted classifier combination method that assigns different weights to classifiers based on their discriminative strengths. We evaluated the described method on previously used datasets, as well as on our in-house dataset, called Trust dataset. Trust dataset is created by using a new subcellular localization hierarchy which merges UniProt Subcellular Location hierarchy and GO Cellular Component hierarchy by applying it on only manual experimental annotations in UniProtKB. We compared our results with five state-of-the-art methods, which are SubCons, LocTree2, CELLO2.5, MultiLoc2, and DeepLoc. Our approach outperformed the others with 59%, 61%, 68% overall Matthews correlation coefficient (MCC) scores on Trust, Golden (SubCons benchmark dataset), Golden-Trust (refined Golden dataset) datasets, respectively where SubCon’s MCC scores were 43%, 53%, and 56%.