Selection of 2'fy rna aptamers against Crimean Congo haemorrhagic fever virus


Thesis Type: Postgraduate

Institution Of The Thesis: Middle East Technical University, Graduate School Of Natural And Applied Sciences, Biological Sciences, Turkey

Approval Date: 2022

Thesis Language: English

Student: FUNDA ZEKİYE ARDIÇ

Principal Supervisor (For Co-Supervisor Theses): Ayşe Gül Gözen

Co-Supervisor: Müslüm İlgü

Abstract:

Crimean-Congo hemorrhagic fever (CCHF) is potentially lethal and mostly infected by ticks. CCHF virus (CCHFV) is a Nairoviridae family member of the Orthonairovirus genus, and in general, cases are reported from Asia, Africa, the Middle East, and Eastern Europe. The fatality rate ranges from 30 to 50% worldwide, but it is reported as 5% by the Turkish Ministry of Health, and it varies between 1.7 and 26.5% for children. ELISA kits used for the detection of infection are time-consuming, expensive, and labor-intensive; because ELISAs detecting IgG and IgM antibodies are produced against the virus, and don’t specifically detect virus presence; these techniques may cause some inaccurate diagnoses, and also they have no reusability. The diagnosis cost will be a lot higher when considering the cost of extra microbiological tests for infectious diseases (Viral hepatitis, typhoid, leptospirosis, and Hantavirus infectious, etc.) that are misdiagnosed with CCHF. As a result, more effective and faster diagnostic technology is required. Aptamers are tiny nucleic acids that can be selected in vitro and have a high affinity and selectivity for their relevant targets. They can contain ssDNA or RNA backbones, vi and they fold into their three-dimensional structure naturally. Aptamers can also be changed to improve their structural stability while maintaining their affinity. Because of these characteristics, they can be used for both therapeutic and diagnostic purposes. CCHFV binding aptamers were successfully selected in this study, and they will be further optimized for CCHF diagnosis Keywords: Aptamer, RNA, SELEX,