Comparative statistical microarray analysis of yeast data under heat shock stress

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Edebiyat Fakültesi, İstatistik Bölümü, Türkiye

Tezin Onay Tarihi: 2014

Öğrenci: DUYGU VAROL

Danışman: VİLDA PURUTÇUOĞLU GAZİ

Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu

Özet:

The microarray technology is one of the widely used experimental methods in biological and biochemical sciences. By this innovation, a number of genes can be analyzed simultaneously by means of statistical methods. Hereby in this study we analyze a new one-channel microarray dataset that is measured to investigate the changes in heat shock stress of yeast. The data that are generated in the Molecular Biology and Biotechnology R-D Center at the Middle East Technical University has not been evaluated yet in different researches. Hence in this study we perform detailed comparative analyses of these measurements and critically assessed the biological findings. For this purpose, in the thesis, we implement the normalization, the detection of differentially expressed genes, multiple testing under different error rates, clustering and the search of gene annotation as well as pathway analyses by comparing the most well-known approaches in each step. Finally, the biological results are evaluated to get new knowledge about the yeast under changes in temperature.