In vitro characterization and nuclear delivery of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) based nanoparticles

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2015

Öğrenci: AYLA ŞAHİN

Danışman: VASIF NEJAT HASIRCI

Özet:

The use of polymeric nanoparticles in life sciences as drug carrier vehicles has been expanding because of their ability to penetrate sites not accessible to larger particles and their large surface area-to-volume ratios that increase their drug release rates. The main objective of this study was to prepare nano sized polymeric particles to deliver active compounds across cell membranes and preferably into the nuclei. This would improve the biostability of macromolecular drugs (growth factors and polynucleotides), and increase their efficacy. Nanocapsules of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) were prepared to study their penetration into the nuclei of Saos-2 human osteosarcoma and L929 cells. The polypeptide based nuclear targeting agent, Nuclear Localization Signal (NLS), was used to improve localization and an anticancer agent, Doxorubicin, was used to study the effectiveness of the PHBV nanoparticles as drug carriers.